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Hierarchical Phase-Contrast Tomography (HiP-CT)



Publication highlights:

Imaging intact human organs with local resolution of cellular structures using hierarchical phase-contrast tomography

Authors: C. L. Walsh, et al.
Journal: Nature Methods
DOI: 10.1038/s41592-021-01317-x
This paper presents a new synchrotron x-ray imaging technique, called Hierarchical Phase-Contrast Tomography (HiP-CT), which is used to span a previously poorly explored scale in our understanding of human anatomy, the micron to whole intact organ scale.

Human Organ AtlasHuman Organ Atlas, see videos at Gallery

The bronchial circulation in Covid-19 pneumonia

Authors: M. Ackermann, et al.
Journal: American Thoracic Society
This paper shows how COVID-19 disrupts the blood vessel network architecture of the lung, specifically we show cases of bronchio-pulmonary shunting in intact COVID-19 lung lobes.

Bronchio-pulmonary shunting in a SARS-CoV-2 infected lungBronchio-pulmonary shunting in a SARS-CoV-2 infected lung

The fatal trajectory of pulmonary COVID-19 is driven by lobular ischemia and fibrotic remodelling

Authors: M. Ackermann, et al.
Journal: eBioMedicine
This paper identifies a link between the damage that severe Covid-19 can inflict on lungs and pulmonary fibrosis, a disease that causes severe scarring of lung tissue.

All Publications

  1. Walsh, C. L., et al. “Imaging intact human organs with local resolution of cellular structures using hierarchical phase-contrast tomography.” Nature methods 18.12 (2021): 1532-1541. DOI: 10.1038/s41592-021-01317-x
  2. Ackermann, M., et al. “The bronchial circulation in COVID-19 pneumonia.” American Journal of Respiratory and Critical Care Medicine 205.1 (2022): 121-125. DOI: 10.1164/rccm.202103-0594IM
  3. Xian, R.P., et al. “A multiscale X-ray phase-contrast tomography dataset of a whole human left lung.” Scientific Data 9, 264 (2022). DOI: 10.1038/s41597-022-01353-y
  4. Jonigk, D., et al. “Pulmonary and Systemic Pathology in COVID-19: Holistic Pathological Analyses.” Dtsch Arztebl Int 119 (2022): 429–35. DOI: 10.3238/arz-tebl.m2022.0231 (German) 10.3238/arztebl.m2022.0231 (English)
  5. Ackermann, M., et al. “The fatal trajectory of pulmonary COVID-19 is driven by lobular ischemia and fibrotic remodelling.” EBioMedicine 85 (2022): 104296. DOI: 10.1016/j.ebiom.2022.104296
  6. Brunet, J., et al. “Preparation of large biological samples for high-resolution, hierarchical, synchrotron phase-contrast tomography with multimodal imaging compatibility.” Nature protocols (2023): 1-27.  DOI: 10.1038/s41596-023-00804-z
  7. Bellier, Alexandre, et al. “Imagerie en contraste de phase par source synchrotron appliquée à l’étude anatomique de la vascularisation artérielle de l’hippocampe.” Morphologie 106.354 (2022): S22-S23. DOI: 10.1016/j.morpho.2022.06.018 (French)
  8. Bellier, Alexandre, et al. “Anatomie multi-échelle par imagerie synchrotron de la vascularisation bronchique dans l’infection à SARS-CoV-2.” Morphologie 106.354 (2022): S38. DOI: 10.1016/j.morpho.2022.06.051 (French)
  9. Ackermann, Maximilian, et al. “Reply to: Intrapulmonary Shunt and Alveolar Dead Space in a Cohort of Patients with Acute COVID-19 Pneumonitis and Early Recovery.” The European respiratory journal: 2202121. (2022). DOI: 10.1183/13993003.02121-2022
  10. Ackermann, Maximilian. “Auf dem Weg zum virtuellen Mikroskop: Synchrotron-basierte dreidimensionale Bildgebung in der Pathologie.” Die Pathologie (2022): 1-6. DOI: 10.1007/s00292-022-01161-6 (German)
  11. Kamp, Jan C., et al. “Novel Insight into Pulmonary Fibrosis and Long COVID.” American Journal of Respiratory and Critical Care Medicine 207.8 (2023): 1105-1107. DOI: 10.1164/rccm.202212-2314LE 
  12. Jain, Yashvardhan, et al. “SenNet + HOA – Hacking the Human Vasculature in 3D.” Kaggle.
  13. Xian, R. Patrick, et al. “A closer look at high-energy X-ray-induced bubble formation during soft tissue imaging.” Journal of Synchrotron Radiation 31.3 (2024): 566-577. DOI: 10.1107/S160057752400290X
  14. Lee, Ju Young, et al. “Protocol for 3D virtual histology of unstained human brain tissue using synchrotron radiation phase-contrast microtomography.” Frontiers in Physics 11 (2024): 1335285.
  15. Submitted: Rahmani, Shahrokh, et al. “Micro to macro scale analysis of the intact human renal arterial tree with Synchrotron Tomography.” (March 2023) DOI: 10.1101/2023.03.28.534566
  16. Submitted: Brunet, Joseph, et al. “Multidimensional Analysis of the Adult Human Heart in Health and Disease using Hierarchical Phase-Contrast Tomography (HiP-CT).” (October 2023). DOI: 10.1101/2023.10.09.561474
  17. Submitted: Brunet, Joseph, et al. “Hierarchical Phase-Contrast Tomography: Advancing High-Resolution Imaging of Intact Human Organs.” (October 2023), Gray’s Anatomy.
  18. Submitted: Yagis, Ekin, et al. “Deep Learning for Vascular Segmentation and Applications in Phase Contrast Tomography Imaging.” (November 2023) DOI: 10.48550/arXiv.2311.13319
  19. Submitted: Mirone, Alessandro,  et al. “Eikonal phase retrieval: Unleashing the fourth generation sources potential for enhanced propagation based tomography on biological samples.” (November 2023) DOI: 10.48550/arXiv.2311.14745
  20. Submitted: Bellier, Alexander, et al. “Micro to macro scale anatomical analysis of the intact human hippocampal arteries with hierarchical phase-contrast synchrotron tomography.” (April 2024)
  21. Submitted: Verleden, Stijn, et al. “Imaging three-dimensional airway morphology in type I Congenital Pulmonary Airway Malformation using Hierarchical Phase-Contrast Tomography.” (May 2024)
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