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HiP-CT
Hierarchical Phase-Contrast Tomography (HiP-CT)

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Imaging intact human organs with local resolution of cellular structures using hierarchical phase-contrast tomography

Authors: C. L. Walsh, P. Tafforeau, W. L. Wagner, D. J. Jafree, A. Bellier, C. Werlein, M. P. Kühnel, E. Boller, S. Walker-Samuel, J. L. Robertus, D. A. Long, J. Jacob, S. Marussi, E. Brown, N. Holroyd, D. D. Jonigk, M. Ackermann and P. D. Lee
Journal: Nature Methods
DOI: 10.1038/s41592-021-01317-x

This paper presents a new synchrotron x-ray imaging technique, called Hierarchical Phase-Contrast Tomography (HiP-CT), which is used to span a previously poorly explored scale in our understanding of human anatomy, the micron to whole intact organ scale. Histology, using optical and electron microscopy, images cells and other structures with sub-micron accuracy but only on small biopsies of tissue from an organ, while clinical CT and MRI scans can image whole organs, but with a resolution only down to just below a millimetre. HiP-CT bridges these scales in 3D, imaging intact organs with ca. 20 micron voxels, and locally down to a few microns. The technique was used to create a “Human Organ Atlas”. We hope this open access Atlas, enabled by the ESRF-EBS, will act as a reference to provide new insights into our biological makeup in health and disease.

Human Organ Atlas
Human Organ Atlas, see videos at Gallery

The bronchial circulation in Covid-19 pneumonia

Authors: M. Ackermann, P. Tafforeau, W. L. Wagner, C. L, Walsh, C. Werlein, M. P. Kühnel, F. P. Länger, C. Disney, A. J. Bodey, A. Bellier, S. E. Verleden, P. D. Lee, S. J. Mentzer and D. D. Jonigk
Journal: American Thoracic Society
DOI10.1164/rccm.202103-0594IM

This paper shows how COVID-19 disrupts the blood vessel network architecture of the lung, specifically we show cases of bronchio-pulmonary shunting in intact COVID-19 lung lobes.

Bronchio-pulmonary shunting in a SARS-CoV-2 infected lung
Bronchio-pulmonary shunting in a SARS-CoV-2 infected lung

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